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. 2019 Jul 11;10:1589. doi: 10.3389/fimmu.2019.01589

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Copyright © 2019 Demars, Lison, Machelart, Van Vyve, Potemberg, Vanderwinden, De Bolle, Letesson and Muraille.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The type of cells infected is dependent on the route of infection. Wild-type C57BL/6 mice (n = 5) were infected intraperitoneally (i.p.) (A), intranasally (i.n.) (B), or intradermally (i.d.) (C) with a dose of 10⁸ (A,B) or 10⁷ (C) CFU of eFluor670 labeled B. melitensis and sacrificed at 2 and 24 h post infection. The spleen (A), lung (B), or footpad lesion (C) were harvested and the cells were isolated and then analyzed by flow cytometry for the expression of eFluor670, CD45, F4/80, CD140a, and CD11b as indicated. Numbers in blue indicate the percentage of eFluor670⁺ cells in each quadrant out of the total cells. These results are representative of three independent experiments.