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. 2019 Jul 11;10:1589. doi: 10.3389/fimmu.2019.01589

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Copyright © 2019 Demars, Lison, Machelart, Van Vyve, Potemberg, Vanderwinden, De Bolle, Letesson and Muraille.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Following intradermal infection with Brucella melitensis, fibroblasts, neutrophils, and macrophages were found to be infected in the footpad lesion. Wild-type C57BL/6 mice were infected intradermally with a dose of 10⁷ CFU of mCherry-B. melitensis. Mice were sacrificed at 24 h post infection and the footpad lesions were collected and analyzed by confocal microscopy for the expression of mCherry and CD140a, LY6G, and F4/80 markers. The panels are color-coded with the text for DAPI, the antigen examined or mCherry-Brucella. Scale bar = 5 μm. Yellow arrowheads indicate the cells infected. The data are representative of two independent experiments.