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. 2019 Jul 17;50:54. doi: 10.1186/s13567-019-0673-8

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Relative expression levels of probable serine carboxypeptidase, cathepsin D, caspase-6, protein S100 and tubulin alpha in the posterior intestine of rainbow trout. Quantitative real-time PCR shows mean relative expression profiles of each selected gene in the posterior intestine of rainbow trout in response to Y. ruckeri biotype 1 (CSF007-82) and biotype 2 (A7959-11) at different time points. Relative gene expression changes in each gene were determined in the exposed and control posterior intestine samples by the CFX Manager Maestro Software in normalized expression mode (∆∆Cq), using elongation factor alpha 1 as a reference at each time point. A Probable serine carboxypeptidase CPVL, B cathepsin D, C caspase 6, D protein S100, and E tubulin alpha. Stars indicate statistically significant differences in the gene expression compared to the control group. Error bars indicate standard deviation (n = 9).