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. 2019 Jul 17;10:211. doi: 10.1186/s13287-019-1316-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Trilineage differentiation potential of established iPSC lines of osteopetrosis patients. a Formation of embryoid bodies from iPSC lines in AggreWells and morphological changes observed during spontaneous differentiation (Patient 1-IPS-P15# 8a-Epi). Cell clumps within the Aggrewell pointed out with arrows. b H&E and immunohistochemical staining of differentiated embryoid bodies on day 21; I—mesenchymal and neuroepithelial area (H&E); ii—mesenchymal and primitive epithelial area (H&E); III—negative OCT4 staining; IV—positive vimentin staining indicating mesodermal differentiation; V—positive synaptophysin staining indicating ectodermal differentiation (Patient 1-IPS-P15# 8a-Epi). c H&E and immunohistochemical staining of teratoma sections. Tissue derivatives indicative of the three germ layers observed, I, III: *epithelial and goblet cells, **connective tissue, ***smooth muscle; II: *epithelial and goblet cells,**peripheral nerve; IV: connective tissue and goblet cells; V: gray matter of central nervous system (I, II H&E, III Mallory Trichrom, IV, V Toluidin) (Patient 1-IPS-P20#11c-SeV)