Fig. 7.

p53 counteracts WWOX-mediated suppression of NCI-H1299 cell growth in nude mice and this antagonism leads to neurodegeneraiton in vivo. a NCI-H1299 cells were transiently overexpressed with p53-DsRed and/or WWOX-ECFP, or EGFP only. Nude mice received subcutaneous injections of these cells twice on both sides of the flanks. Tumor sizes were measured daily. WWOX suppressed tumor growth, and the suppression was abolished by p53. A representative data is shown from two experiments. b Mice were sacrificed on day 86. Spleen enlargement occurred in all mice except in the mouse harboring WWOX-expressing NCI-H1299. c Protein aggregates were found for pERK, BACE, APP, Aβ, NFT and α-tubulin in the brain of mouse inoculated with p53/WWOX-expressing NCI-H1299 (top panel; nonreducing gels). APP was degraded. Under reducing conditions (bottom panel), aggregation of BECN-1, neurofilament NF-M/H, and AIF is shown. d Similar results were observed for the expression of BACE, APP, Aβ, BECN-1, and αSynuclein in the mouse lung