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. 2019 Jul 18;19:48. doi: 10.1186/s12896-019-0545-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Assessing the structure and function of cysteine-substituted flagella. a Fluorescent staining of S. Typhimurium shows production and structural appearance of the respective flagella. Images included from three independent experiments. b Measurements of helical curvature (μM^− 1) and twist (μM^− 1) from each modified flagella compared to theoretical helical structures (red symbols). Multiple measurements were taken from both attached and detached filaments. Data (n ≥ 6) from two independent experiments. c Soft-agar motility assay depicting the effect of cysteine substitutions on the swimming motility of S. Typhimurium. Image representative of three independent experiments