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. 2019 Jul 18;19:48. doi: 10.1186/s12896-019-0545-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Crosslinking and stability of cysteine-substituted flagella. a Extracted filaments subjected to SDS-PAGE with and without β-mercaptoethanol (β-ME) reduction signifying the presence of disulfide bonds in all three cysteine-substituted flagella. Image representative of three independent experiments. b Thermal and acidic filament stability assessed by Native-PAGE following treatment with 50 mM HCl or heating at 60 °C for 10 min compared to untreated (UT). Image representative of two independent experiments. c Densitometry quantitation of replicate Native-PAGE gels as shown in b. Data included from two independent experiments with standard deviation shown