Fig. 6.
Long-term temperature stability of FliCQ62C,Q97C in solution. a Solutions of extracted WT and FliCQ62C,Q97C flagella filaments were incubated at 4 °C, 25 °C, 37 °C, and 42 °C for 7 days, and 3 ng was subjected to Native-PAGE to assess filament stability, depolymerization, and degradation. Image representative of two independent experiments. DP indicates an 80 °C depolymerized WT control sample. b Densitometry quantification of filaments (black arrow) and degraded / depolymerized protein. Data includes measurements from two independent experiments with standard deviation shown. Asterisk indicates statistically-significance (p < 0.05) difference compared to WT FliC as determined by 2-way ANOVA with Tukey’s multiple comparisons test