Fig. 3.

Reduced pyramidal neurons in the upper cortical layer of LIMK KO mice. a and b Sample coronal brain sections stained for the late-born neuronal marker Brn2 (red) and BrdU (green) injected at E14.5, showing co-location of Brn2⁺ and BrdU⁺ neurons (yellow) in the upper layer of P0 cortex. c Summary graph showing reduced Brn2⁺/BrdU⁺ neurons in LIMK1 KO, LIMK2 KO and LIMK1/2 DKO mice. WT n = 6, LIMK1 KO n = 5, LIMK2 KO n = 5, LIMK1/2 DKO n = 3. Data are presented as mean ± SEM. *p < 0.05, one-way ANOVA, Dunnett’s test. Scale bars, 50 μm