Fig. 7.

Altered cell cycle state in the telencephalon of LIMK KO mice. a Pregnant mice were injected with BrdU at E13.5 for 24 h and the embryos of the control and various LIMK KO were then dissected at E14.5 and processed for immunostaining for BrdU (green), Ki67 (red) and DAPI (blue). b Magnified views of the co-localization of BrdU⁺/Ki67⁺ cells. c-e Summary graphs of the percentages of BrdU⁺/Ki67⁺(c) and BrdU⁺/Ki67⁻ (d) cells relative to the total BrdU⁺ cells showing a significantly decreased number of cells staying in the active phases of the cell cycle, as indicated by reduced BrdU⁺/Ki67⁺ cells and increased BrdU⁺/Ki67⁻ cells, in LIMK KO mice compared to the control littermates. Summary graph showing that the number of total Ki67⁺ cells (e) was also decreased in the dorsal telencephalon in the LIMK KO mice compared to the control littermates. Control, n = 6; LIMK1 KO, n = 3; LIMK2 KO, n = 5; LIMK1/2 DKO, n = 3. Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA, Dunnett’s test. *p < 0.05, **p < 0.01. Scale bars, 50 μm