Fig. 8.

Reduced proliferation of intermediate neural progenitors in LIMK KO mice. a Pregnant mice were injected with BrdU at E13.5 for 24 h and the embryos of the control and various LIMK KO were then dissected at E14.5 and processed for immunostaining for Sox2 (purple), Tbr2 (red) and BrdU (green). b-d Summary graphs of the number of Sox2⁺/BrdU⁺(b), Tbr2⁺/BrdU⁺(c) and BrdU⁺/Sox2⁻/Tbr2⁻ cells (d).Although the numbers of newborn radial glial cell (Sox2⁺/BrdU⁺), intermediate progenitor (Tbr2⁺/BrdU⁺) and neurons (BrdU⁺/Sox2⁻/Tbr2⁻ cells) all showed a trend of decrease in LIMK KO mice compared to the control littermates, only the reduction of intermediate progenitors (Tbr2⁺/BrdU⁺) showed a significant difference. Control, n = 6; LIMK1 KO, n = 3; LIMK2 KO, n = 5; LIMK1/2 DKO, n = 3. Data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA, Dunnett’s test. *p < 0.05, **p < 0.01. Scale bars, 50 μm