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. 2019 Jul 17;20:25. doi: 10.1186/s12860-019-0207-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Age-dependent expression of Cited 2 in mice heart tissues and CSCs. a. RT-PCR for Cited2 mRNA expression in Young (2–3 months), Middle (4–6 months) and Old (20–22 months) mice heart tissues. b. The analysis of RT-PCR for Cited2 mRNA expression. c. DD-PCR for Cited2 mRNA expression in Young (2–3 months) and Old (20–22 months) CSCs. d-g. Immunoblotting of CITED2 protein levels in lentivector transduction groups and CSCs groups. OE represented Old CSCs transducted with lentiviral particles of Cited2-cDNA, KD represented Young CSCs transducted with lentiviral particles of Cited2-shRNA3, the control was transducted with empty lentivector. Data were from three independent experiments and presented as mean ± SD. Statistical analysis was implemented by GraphPad Prism 5, *p < 0.05, ** p < 0.01, and *** p < 0.001, Old-CSCs contrast to Young-CSCs