Fig. 3.

The affect of Cited2 knockdown on differentiation in Young cardiac stem cells. a: DD-PCR of Cited2 mRNA expression for lentivector transduction. The control was transducted with empty lentivector. Knockdown represented Young CSCs transducted with lentiviral particles of Cited2-shRNA1, Cited2-shRNA2 and Cited2-shRNA3; Data were from three independent experiments and presented as mean ± SD.Statistical analysis was implemented by GraphPad Prism 5, *p < 0.05, ** p < 0.01, and *** p < 0.001, contrast to control. b: The differentiation efficiency of CSCs from Cited2 knockdown and negative control group. The percentage % of cells differentiated into cardiomyocytes was calculated cardiomyocytes marker cells among total GFP cells based on a mean of five independent experiments. Statistical analysis was implemented by GraphPad Prism 5, *p < 0.05, ** p < 0.01, and *** p < 0.001, contrast to control. c-e: Immunofluorescence staining (red) of cardiomyocytes-specific marker-cTnI; smooth muscle marker-α-SMA and enodthelial cell marker-VWF; transfection CSCs staining with GFP; then merged images. Each experiment is repeated five times, and the picture represents a single result. Knockdown represented Young CSCs transfected with lentiviral particles of Cited2-shRNA3; The control was transducted with empty lentivector. GFP-green is represented lentivirus vector. Scale bars, 50 μm