Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Sequences of Cited2-shRNA and the primers
| Primer | Forward primer 5′ → 3′ | Reverse primer 5′ → 3′ |
|---|---|---|
| Cited2 (for dd PCR and RT-PCR) | AAATCGCAAAGACGGAAGGA | TGAACTGGGAGTTGTTAAACCT |
| Cited2-shRNA1 | TGGCAGACCATATGATGGCCATGAA | |
| Cited2-shRNA2 | CCCACAACCACTACATGCCGGATTT | |
| Cited2-shRNA3 | GTCAGCTGTTGACTCGGTTAA | |
| Cited2-cDNA | tatcgaattc(EcoRI)ATGGCAGACCATATGATGGCCATGAAC | tatcggatcc(BamHI)ACAGCTGACTCTGCTGGGCTGCT |
| GAPDH | GCTGCCCAGAACATCATCCCT | TGAAGTCGCAGGAGACAACC |
