Fig. 4. Scaling relations between the CF and system parameters revealed by combining experiment, simulation, and theory. The red and blue lines are the fitting lines between the experimental/simulation results and the scaling laws revealed by the theoretical EK and EN limits, respectively. (a) Scaling relation between the CF and Vcn by experiment. 1 μM fluorescent ssDNA in 10 mM KCl was used as the analyte. VHL was set to 10 V. (b) Scaling relation between the CF and Vcn by simulation. 0.1 nM default particle in 1 mM KCl was used as the analyte. VHL was set to 20VT. (c) Scaling relation between the CF and the initial analyte concentration by experiment. Fluorescent ssDNA with varied initial concentrations in 10 mM KCl was used as the analyte. VHL was set to 10 V. (d) Scaling relation between the CF and the initial analyte concentration by simulation. Default particle with varied initial concentrations in 1 mM KCl was used as the analyte. VHL was set to 20VT. (e) Scaling relation between the CF and the buffer concentration by experiment. 1 μM fluorescent ssDNA in KCl solutions of varied concentrations was used as the analyte. VHL was set to 10 V, and Vcn was set to 5 V. (f) Scaling relation between the CF and the buffer concentration by simulation. 0.1 nM default particle in KCl solutions of varied concentrations was used as the analyte. VHL was set to 20VT, and Vcn was set to 30VT.
