Table 3.
Retention times for Kupchan fractions in HPLC.
| Peak | (x) RT (min) | Width (min) | Area (mAU∗s) | (y) Height (mAU) | Area (%) |
|---|---|---|---|---|---|
| ZRC | |||||
| 1 | 2.872 | 0.1155 | 61.05810 | 7.38034 | 1.7119 |
| 2 | 3.167 | 0.0989 | 240.89647 | 37.04975 | 6.7541 |
| 3 | 3.373 | 0.1350 | 270.91974 | 37.48990 | 7.5958 |
| 4 | 3.730 | 0.1115 | 2536.17334 | 337.99835 | 71.1074 |
| 5 | 4.328 | 0.1290 | 115.58913 | 13.35508 | 3.2408 |
| 6 | 4.621 | 0.1279 | 15.72073 | 1.79944 | 0.4408 |
| 7 | 5.301 | 0.1223 | 129.75060 | 15.88849 | 3.6379 |
| 8 | 5.654 | 0.1332 | 33.90483 | 3.83241 | 0.9506 |
| 9 | 14.339 | 0.1536 | 101.36829 | 9.34081 | 2.8421 |
| 10 | 14.693 | 0.2510 | 61.30116 | 3.50620 | 1.7187 |
|
| |||||
| ZRFH | |||||
| 1 | 2.879 | 0.1101 | 32.03850 | 4.44205 | 1.4512 |
| 2 | 3.183 | 0.0999 | 105.94155 | 15.86805 | 4.7988 |
| 3 | 3.377 | 0.0904 | 60.97616 | 9.96817 | 2.7620 |
| 4 | 3.479 | 0.0849 | 35.27485 | 6.24909 | 1.5978 |
| 5 | 3.746 | 0.1102 | 1726.87097 | 233.63256 | 78.2214 |
| 6 | 4.336 | 0.1189 | 71.96671 | 9.13256 | 3.2598 |
| 7 | 5.318 | 0.1276 | 102.20798 | 11.97620 | 4.6297 |
| 8 | 14.451 | 0.1441 | 72.39410 | 7.21304 | 3.2792 |
|
| |||||
| ZRFD | |||||
| 1 | 2.902 | 0.1293 | 36.92966 | 4.47213 | 1.4285 |
| 2 | 3.185 | 0.1008 | 440.68698 | 64.46280 | 17.0464 |
| 3 | 3.372 | 0.0900 | 94.99347 | 15.39953 | 3.6745 |
| 4 | 3.482 | 0.0933 | 56.08873 | 9.30652 | 2.1696 |
| 5 | 3.743 | 0.1033 | 1624.33984 | 232.98772 | 62.8316 |
| 6 | 3.927 | 0.0924 | 206.64229 | 32.40942 | 7.9932 |
| 7 | 4.335 | 0.1152 | 41.74596 | 5.58815 | 1.6148 |
| 8 | 5.304 | 0.1243 | 36.92151 | 4.52034 | 1.4282 |
| 9 | 14.357 | 0.1347 | 46.87906 | 5.07797 | 1.8133 |
|
| |||||
| ZRFM | |||||
| 1 | 2.891 | 0.1349 | 63.85921 | 7.53434 | 1.6574 |
| 2 | 3.185 | 0.1042 | 525.79871 | 74.60051 | 13.6468 |
| 3 | 3.374 | 0.0883 | 109.30883 | 18.13418 | 2.8370 |
| 4 | 3.485 | 0.0934 | 75.00216 | 12.25811 | 1.9466 |
| 5 | 3.745 | 0.1082 | 2825.35791 | 386.82672 | 73.3303 |
| 6 | 4.336 | 0.1164 | 70.74014 | 9.34014 | 1.8360 |
| 7 | 5.306 | 0.1243 | 61.64240 | 7.46639 | 1.5999 |
| 8 | 14.459 | 0.1800 | 121.21284 | 9.81946 | 3.1460 |
|
| |||||
| ZRWB | |||||
| 1 | 2.874 | 0.1237 | 35.44300 | 4.41159 | 3.1619 |
| 2 | 3.174 | 0.1107 | 41.06397 | 5.46061 | 3.6634 |
| 3 | 3.374 | 0.1285 | 43.36354 | 4.61984 | 3.8685 |
| 4 | 3.732 | 0.1124 | 830.25043 | 109.60705 | 74.0683 |
| 5 | 4.324 | 0.1217 | 36.01653 | 4.53515 | 3.2131 |
| 6 | 5.28 9 | 0.1306 | 53.58618 | 6.15428 | 4.7805 |
| 7 | 14.342 | 0.1273 | 48.23082 | 5.29576 | 4.3028 |
| 8 | 14.605 | 0.2202 | 32.97150 | 2.06582 | 2.9415 |
Ordinate(x) = absorbance intensity; Abscissa (y) = retention time; ZR=Z. zanthoxyloides LZT (root); ZRC=crude extract of ZR; ZRFH= Z. zanthoxyloides hexane fraction; ZRFD= Z. zanthoxyloides dichloromethane fraction; ZRFM= Z. zanthoxyloides methanol fraction; ZRWB= Z. zanthoxyloides butanol fraction; NA= not applicable.