Table 4.
Effect of Kupchan fractions on cell viability of T. brucei and human cell lines.
| FRACTIONS | MEAN IC50 ± SE (μg/ml) | SI | |||
|---|---|---|---|---|---|
| T. brucei | Jurkat | Chang liver | Jurkat | Chang liver | |
| ZRC | 3.41±0.06 | NA | NA | NA | NA |
| ZRFD | 5.70 ± 0.03 | 52.04 ± 0.04 | 89.93 ± 0.03 | 9.13 | 15.78 |
| ZRFH | 13.26 ± 0.11 | NA | NA | NA | NA |
| ZRFM | 3.89 ± 0.06 | 68.15 ± 0.05 | 93.84 ± 0.03 | 17.52 | 24.12 |
| ZRWB | 4.02 ± 0.06 | 25.28 ± 0.05 | 375.58 ± 0.07 | 6.29 | 93.43 |
| DA | 0.54 ± 0.05 | NA | 39.72 ± 0.03 | NA | 73.56 |
| DX | NA | 0.27 ± 0.28 | 145.22 ± 0.02 | NA | NA |
Mean IC50 and standard errors (SE) were calculated from three distinct experiments. SI was calculated as the ratio of IC50 in Jurkat or Chang liver cells to the IC50 in T. brucei. ZR=Z. zanthoxyloides, root; ZRC=crude extract of ZR; ZRFH= Z. zanthoxyloides hexane fraction; ZRFD= Z. zanthoxyloides Dichloromethane fraction; ZRFM= Z. zanthoxyloides methanol fraction; ZRWB= Z. zanthoxyloides butanol fraction; DA=Diminazene aceturate, (an antitrypanosomal drug); DX=Doxorubicin, (an antileukaemia drug); SI= selectivity index; NA=Not applicable (only ZRFD, ZRFM, and ZRWB were considered for further analysis due to their promising IC50 values against T. brucei).