Table 1.
Real-Time Polymerase Chain Reaction Primers and Predicted Product Sizes a
| Gene | Sequence (5′-3′) | Size, base pairs |
|---|---|---|
| SOX9 | Sense: AATCTCCTGGACCCCTTCATG | 60 |
| Antisense: GGCGGACAGGCCCTTCT | ||
| COL2A1 | Sense: AAGAAGGCTCTGCTCATCCAGG | 124 |
| Antisense: TAGTCTTGCCCCACTTACCGGT | ||
| ACAN | Sense: CCTGAACGACAAGACCATCGA | 101 |
| Antisense: TGGCAAAGAAGTTGTCAGGCT | ||
| COL1A1 | Sense: AAGAACCCAGCTCGCACATG | 82 |
| Antisense: GGTTAGGGTCAATCCAGTAGTAACCA | ||
| MMP3 | Sense: TGGTCCAGGAGATGAAGACC | 109 |
| Antisense: TGGCATCAAGGGATAAGGAA | ||
| ADAMTS5 | Sense: CTCCCATGACGATTCCAA | 85 |
| Antisense: AATGCTGGTGAGGATGGAAG | ||
| PRG4 | Sense: AGAAAACCCGATGGCTATGA | 106 |
| Antisense: TCGCCCATCAGTCTAAGGAC | ||
| 18S rRNA | Sense: TCGAGGCCCTGTAATTGGAA | 104 |
| Antisense: GCTATTGGAGCTGGAATTACCG |
a
ACAN, aggrecan; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; COL1A1, collagen type I α1; COL2A1, collagen type II α1; MMP, matrix metalloproteinase; PRG, proteoglycan; SOX9, SRY-related HGMG box containing gene 9; 18S rRNA, 18S ribosomal RNA.