Figure 6.

Accumulation of PvRXLR131 in Arabidopsis suppressed BR and ER signalling. (A) Overexpression of PvRXLR131‐GFP leads to a reduced response to BL. Hypocotyl length of at least 40 seedlings was measured. (B) Accumulation of PvRXLR131 alters the expression of BR‐responsive genes. The expression level of two BR down‐regulated genes, CPD and DWF4, was increased and that of the up‐regulated gene, Saur‐AC1, was reduced. Each data point represents means ± standard deviations (SDs) from three biological replicates, values with different letters show significant differences between mean values at P = 0.05 (LSD). U‐box gene (At5g15400) was used to normalize the data. (C) Comparison of MPK activity in PvRXLR131‐transgenic, GFP‐transgenic and Col‐0 Arabidopsis. Total proteins were extracted from 2‐week‐old seedlings; equal protein amounts were separated using SDS‐PAGE electrophoresis, and immunoblots were conducted using an anti‐p44/42‐ERK antibody. A Ponceau‐S stained RuBisCO large subunit (rbcL) served as a loading control.