Figure 2.
Symptoms of Alternaria brassicicola infection are suppressed in Arabidopsis plants with reduced PRX33/PRX34 mRNA levels. Leaves of A. brassicicola‐infected Col‐0, prx33 knockdown mutant, rbohd knockout mutant, TRV‐GFP and TRV‐PRX plants are shown 10 days after inoculation. Five‐ to six‐week‐old Arabidopsis plants (whole rosettes) were spray inoculated with A. brassicicola conidial suspension at a concentration of 5 × 105 conidia/mL distilled water. Detached leaves in middle positions (leaf levels 5–8) were evaluated. Results are presented as the chlorotic and necrotic leaf area compared with the total surface area of leaf blades analysed by ImageJ (1 is equal to 100% leaf surface), and represent the means of two experiments (n = 30 for each genotype/treatment) ± standard error (SE). Different letters indicate statistically significant differences between genotypes/treatments using Tukey’s post hoc test. Increased symptoms of A. brassicicola on leaves of the rbohd mutant are included as a reference.