Figure 6.
Transcript levels of PRX33 and PRX34 genes in nho1 and jar1 T‐DNA insertion lines relative to wild‐type plants. The level of gene expression was analysed before and after inoculation with Alternaria brassicicola [24 h after inoculation (hai)]. Dysfunctions in NHO1 and JAR1 activity affect PRX33 and PRX34 mRNA levels. Five‐ to six‐week‐old Arabidopsis plants (whole rosettes) were spray inoculated with A. brassicicola conidial suspension at a concentration of 5 × 105 conidia/mL distilled water. The results show the average of two experiments, each comprising three biological samples (each sample composed of a pool of three Arabidopsis rosettes), analysed in three technical replicates. Statistical analysis was performed using Student’s t‐test. Asterisks indicate statistically significant differences (*α = 0.05, **α = 0.01, ***α = 0.001).