Figure 8.
Hydrogen peroxide (H2O2) production in wild‐type (Col‐0) and erecta T‐DNA insertion line Arabidopsis leaves. The accumulation of H2O2 was visualized by 3,3′‐diaminobenzidine (DAB) staining. Five‐ to six‐week‐old Arabidopsis plants (whole rosettes) were spray inoculated with Alternaria brassicicola conidial suspension at a concentration of 5 × 105 conidia/mL distilled water. The staining was carried out with mock‐inoculated and A. brassicicola‐infected plants [48 h after inoculation (hai)]. Leaves of the A. brassicicola‐infected erecta mutant show markedly reduced accumulation of H2O2. The detection was repeated twice using 15 plants for each treatment. Representative leaves are shown.