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. 2018 Oct 11;20(2):194–210. doi: 10.1111/mpp.12748

Figure 5.

Figure 5

Co‐expression of Potato virus X (PVX) P25 together with Plum pox virus (PPV) helper component‐proteinase (HC‐Pro) promotes endoplasmic reticulum (ER) stress. Nicotiana benthamiana leaves were infiltrated with Agrobacterium cultures expressing T7‐tagged P25, HC‐Pro or green fluorescent protein (GFP) alone or a combination of cultures expressing T7‐tagged P25 plus HC‐Pro. Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) analysis of the expression of ER stress marker genes in N. benthamiana leaves at 3 [luminal binding protein (BiP), bZIP60, heat shock protein 90‐2 (HSP90‐2), S‐phase kinase‐associated protein 1 (SKP1) and calreticulin (CRT)] or 6 days post‐infiltration (calmodulin, CAM), as indicated. Expression of the 18S rRNA gene served as a control. Data represent the means ± standard errors of three replicates, each consisting of three plants that received the same treatment. Statistically significant differences between means were determined by employing Scheffé’s multiple range test, except for CAM, where these differences were determined by T3 Dunnett´s test. Different letters indicate significant differences at P < 0.05.