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. 2019 Feb 8;20(4):599–608. doi: 10.1111/mpp.12779

Figure 2.

Figure 2

Effects of the lysine residues of AvrPiz‐t on the activation of Piz‐t‐mediated resistance. (a) Effects of spray inoculation of NPB‐Piz‐t:HA transgenic rice and NPB wild‐type rice with different Magnaporthe oryzae RB22 isolates. Two transformants of the M. oryzae isolate RB22 harbouring the LF‐AvrPiz‐t or AvrPiz‐t constructs were used for inoculation, whereas the non‐transformed M. oryzae RB22 was used as a control. Infected leaves were assessed at 6 days post‐inoculation (dpi). (b) Effects of punch inoculation of NPB‐Piz‐t:HA transgenic rice and NPB wild‐type rice with different M. oryzae RB22 isolates. Magnaporthe oryzae RB22‐LF‐AvrPiz‐t or RB22‐AvrPiz‐t transformants from the spray inoculation were used. Six‐week‐old plants were inoculated with an M. oryzae spore suspension. Infected leaves were assessed at 9 dpi.