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. 2019 Feb 8;20(4):599–608. doi: 10.1111/mpp.12779

Figure 3.

Figure 3

Effects of the lysine residues on AvrPiz‐t‐induced Piz‐t protein accumulation during Magnaporthe oryzae infection. NPB‐Piz‐t:HA transgenic plants were inoculated with M. oryzae isolate RB22, RB22‐AvrPiz‐t or RB22‐LF‐AvrPiz‐t. Immunoblots with anti‐HA were conducted to detected Piz‐t protein accumulation at different time points after inoculation (hpi, hours post‐inoculation). Endogenous rice HSP90 detected by anti‐HSP was used as an internal control, and NPB wild‐type samples served as a negative control (last lane). Protein levels were measured by Image Lab software on the basis of the band intensity and were then normalized with the internal control.