Figure 5.
Effects of the lysine residues of AvrPiz‐t on the suppression of rice basal resistance. (a) Effects of punch inoculation of NPB‐LF‐AvrPiz‐t:HA and NPB‐AvrPiz‐t:HA transgenic plants with Magnaporthe oryzae isolate RB22. Two independent plants of each transgenic line were used. NPB wild‐type plants served as a control. Six‐week‐old plants were inoculated with an M. oryzae spore suspension of 5 × 105 spores/mL. Phenotype leaves were assessed at 9 days post‐inoculation (dpi). (b) Relative fungal growth in the photographed leaves was measured by DNA‐based real‐time PCR to quantify the amount of DNA of M. oryzae using two sets of primers specific for M. oryzae Pot2 and rice Ubiquitin. Values are means ± standard error (SE) (n = 3). One‐way analysis of variance (ANOVA) test with Tukey’s method was conducted. Means followed by different letters are significantly different (P < 0.05).