Figure 2.

Subcellular localization of GhDSC1 in Nicotiana benthamiana. (A) Structure of nuclear localization signals (NLS) and mutations in GhDSC1. NLS1 and NLS2 represent two nuclear localization signals in GhDSC1. GhDSC1^NLS1, GhDSC1^NLS2, GhDSC1^NLS1+2 represent mutation of NLS1, NLS2 and deletion of NLS1 and NLS2 together, respectively. (B) Subcellular localization of GhDSC1 and the mutant alleles were determined by transient expression of the C‐terminally green fluorescent protein (GFP)‐tagged proteins in N. benthamiana leaves. The fluorescence was scanned by a Leica TCS SP8 confocal microscopy system using × 200 magnification with a excitation at 488 nm and emission at 510 nm. The empty vector 35S::GFP (GFP) was a negative control. Nuclei were stained using 4',6‐diamidino‐2‐phenylindole (DAPI).