Figure 3.
Expression of GhDSC1 in response to Verticillium dahliae infection and hormone signalling in cotton. (A) Expression analysis of GhDSC1 in four cotton cultivars over time after inoculation with V. dahliae strain Vd991 by Reverse Transcription‐quantitative Polymerase Chain Reaction (RT‐qPCR). Three‐week‐old cotton plants, including two resistance cultivars (cv. Zhongzhimian No. 2 and cv. AA085) and two susceptible cultivars (cv. Junmian No. 1 and cv.Jimian No. 11), were inoculated with conidial suspension (5 × 106 conidia/mL) and harvested at the respective time points. Different letters indicate significant differences at P < 0.01 based on Tukey's HSD. (B–D) GhDSC1 expression in response to the four hormone treatments. The transcript levels of GhDSC1 were detected in 3‐week‐old cotton plants (cv. Zhongzhimian No. 2) treated with the (B) MeJA, (C) ETH, (D) SA and (E) ABA. Relative expression analyses of GhDSC1 by RT‐qPCR was performed using the cotton 18S gene as a reference using the comparative threshold 2‐ΔΔCT method, and relative expression was compared with expression levels in cotton plants that were treated with sterile water (Mock). The values shown represent averages of three independent biological replicates of three plants each. Error bars were calculated based on three biological replicates using standard deviation; asterisks (∗) and double asterisks (∗∗) represents statistical significance of P < 0.05 and P < 0.01, respectively, according to an unpaired Student's t‐tests of each of treatment groups compared with control (Mock).