Figure 5.
GhDSC1 complements Verticillium wilt resistance in an Arabidopsis thaliana dsc1 mutant. (A) Verticillium wilt phenotype A. thaliana line dsc1, a T‐DNA mutant of DSC1, which is orthologous to GhDSC1. (B) Identification of Verticillium wilt resistance of ectopic transformants in which GhDSC1 was introduced into the dsc1 mutant. Three‐week‐old transgenic (complement transformants) lines (EC1, EC2 and EC3) were subjected to a root‐dip inoculation in a suspension of 5 × 106 conidia/mL of Verticillium dahliae, strain Vd991. Verticillium wilt symptoms were assessed 21 days after inoculation. Sterile water was used in controls (Mock). (C) Quantification of V. dahliae biomass in transgenic GhDSC1 lines of the dsc1 mutants by quantitative Polymerase Chain Reaction (qPCR). Error bars represent standard errors of three biological replicates, asterisks (∗) and double asterisks (∗∗) represents statistical significance of P < 0.05 and P < 0.01, respectively, according to unpaired Student's t‐tests between dsc1 mutants and EC plants compared with the wild‐type (Col‐0).