Figure 2.

Next‐generation sequencing (NGS) in DCLi and DCLi crossed plants. (A–C) Northern blot analysis of DCLi and DCLi crossed lines agroinfiltrated with green fluorescent protein (GFP) and GFPhp constructs; 21‐, 22‐ and 24‐nucleotide (nt) small RNAs were monitored. U6 was used as internal control. (D, E) Distribution of 20–24‐nt and 26–34‐nt small RNAs in wild‐type (WT), DCL1.13i, DCL2.11i, DCL3.10i, DCL4.9i, DCL1.13(x)2.11i, DCL2/4.5i and DCL3.10(x)2/4.5i. (F, H, I) 21–24‐nt micro‐RNA (miRNA) and trans‐acting small interfering RNA (tasiRNA) levels in the sequenced plant lines. (G) Validation of the bioinformatics analysis with quantitative polymerase chain reaction (qPCR) of miR159, miR166, miR168, miR396 and miR397. [Colour figure can be viewed at wileyonlinelibrary.com]