Figure 2.

Biofilm formation analysis of the Xanthomonas citri ssp. citri (Xcc) hupB mutant strain. (A) Microtitre plate adhesion assay. Crystal violet absorbance (Abs_570 nm) to OD₆₀₀ ratio is represented for each strain: wild‐type strain (Xcc 306), null mutant strain (hupB) and the complemented strain (c‐hupB). Error bars indicate standard error of the mean of three independent experiments. (B) Biofilm structures after 4 days of inoculation: projections in the x–y plane were obtained through confocal laser scanning microscopy (CLSM) (40× magnification); z‐axis‐projected images are shown below (scale bars, 5 μm).