Figure 3.

FLS2 function is essential for the flg22‐induced resistance to Fusarium graminearum (Fg) in Arabidopsis. (A) Real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) evaluation of fold induction of WRKY29 expression in flg22‐treated and Fg‐inoculated leaves of wild‐type (WT) and fls2 mutant plants, relative to expression in the corresponding mock‐inoculated leaves. Gene expression was monitored 24 h post‐treatment, with the expression of At1g07940 providing the control. All values are the means ± standard error (SE) (n = 5). Different letters above the bars indicate values that are significantly different from each other (P < 0.05; Tukey’s test). (B) Leaf disease index in WT accession Columbia and fls2 mutant leaves treated with 50 ng of flg22 (+) or as control with water (–). Fungal inoculation was conducted 24 h after flg22 treatment and disease severity was monitored at 5 days post‐inoculation (dpi) with the fungus. All values are the means ± SE (n = 50). Different letters above the bars indicate values that are significantly different from each other (P < 0.05; Tukey’s test). (C) Leaf disease index in Fg‐inoculated WT accession Columbia plant, PR1‐flg22 transgenic line #2 in the FLS2 background, the fls2 mutant and two independent PR1‐flg22 transgenic lines in the fls2 mutant background. Disease severity was monitored at 5 dpi. All values are the means ± SE (n = 50). Different letters above the bars indicate values that are significantly different from each other (P < 0.05; Tukey’s test).