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. 2019 Feb 6;20(5):626–640. doi: 10.1111/mpp.12781

Figure 5.

Figure 5

Ectopic expression of WRKY29 in wheat promotes resistance to Fusarium head blight. (A) Top: reverse transcription‐polymerase chain reaction (RT‐PCR) demonstration of WRKY29 expression in leaves of wheat cv. Bobwhite (Bw) and three independent Ubi:WRKY29 transgenic wheat lines in which the Arabidopsis WRKY29 coding sequence is expressed from the maize Ubi promoter. Middle: FHB disease severity in wheat cv. Bw and Ubi:WRKY29 lines in the Bw background. Disease progression was monitored at 5, 9, 15 and 21 days post‐inoculation (dpi) of spikes. All values are the means ± standard error (SE) (n = 12). Asterisks above the bars indicate values that are significantly different from the WT for that particular time point (P < 0.05; t‐test). Bottom: photograph showing disease spread in a representative spike from wheat cv. Bw and the Ubi:WRKY29 lines. Photographs were taken at 21 dpi. (B) Real‐time PCR analysis of the DNA content of the Fusarium graminearum (Fg) NahG gene relative to the wheat TUB2 gene in wheat spikes at 2 dpi with Fg. All values are the means ± SE (n = 3). Asterisks above the bars indicate values that are significantly different from Bw (P < 0.05; t‐test). (C) Deoxynivalenol (DON) content (µg/g seed) in Fg‐inoculated wheat cv. Bw and the Ubi:WRKY29 transgenic wheat lines. All values are the means ± SE (n = 3). Each sample included 3–5 g of seeds collected from two to three spikes derived from separate plants. Asterisks above the bars indicate values that are significantly different from Bw (P < 0.05; t‐test).