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. 2019 Jan 30;20(4):575–588. doi: 10.1111/mpp.12776

Figure 2.

Figure 2

Activation of Rx1 triggers a rapid reactive oxygen species (ROS) burst. (A) 3,3′‐Diaminobenzidine (DAB) staining of DEX::CP105 and DEX::CP106 agroinfiltrated Rx1:4xHA leaves at three time points after dexamethasone (DEX) induction. Inset shows a close‐up of a representative CP106/Rx1 sector. (B) Box‐and‐whisker plots showing the cumulative production of ROS, measured using a plate reader‐based assay in arbitrary units (AU). Outliers are indicated by individual dots. Left panel shows Rx1:4xHA plants in which DEX::CP105 or DEX::CP106 was expressed. The other three panels show the co‐expression of the coat protein (CP) constructs with green fluorescent protein (GFP)‐tagged Rx1 wild‐type or a nuclear‐localized (Rx1‐NLS) or nuclear‐excluded (Rx1‐NES) version. Letters depict significantly different (P < 0.05) categories, as determined by Tukey’s honestly significant difference (HSD) test. Each column consists of cumulative measurements of 9–15 discs. (C) Kinetics of ROS production over time following DEX treatment of the samples depicted in (B).