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. 2018 Dec 6;20(3):392–409. doi: 10.1111/mpp.12764

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© 2018 The Authors. Molecular Plant Pathology published by BSPP and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The Potato virus A (PVA) proteins helper component proteinase (HCPro), cytoplasmic inclusion protein (CI), nuclear inclusion protein a (NIa) and coat protein (CP) are present in the ribosome‐enriched P170K samples. (A) Protein pattern of P170K samples in silver‐stained sodium dodecylsulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) showed enrichment of proteins below 55 kDa when compared with the total protein sample. (B) The presence of ribosomes in P170K samples was verified by western blotting with antisera recognizing RPL18 from the 60S subunit (anti‐FLAG) and RPS6 from the 40S subunit. (C) PVA proteins CI, NIa and CP were detected with their corresponding antisera, and HCPro with anti‐RFP antiserum recognizing RFP‐tagged HCPro. [Colour figure can be viewed at wileyonlinelibrary.com]