Figure 4.

The effect of Southern rice black‐streaked dwarf virus (SRBSDV) infection on OEP10 transgenic plants expressing the Rice black‐streaked dwarf virus (RBSDV) P10 protein. (a) The visual appearance of mock‐ and SRBSDV‐infected plants. White bar represents 5 cm. (b) The relative expression levels of SRBSDV genes in SRBSDV‐infected plants as assessed by quantitative reverse transcription‐polymerase chain reaction (RT‐qPCR). (c) SRBSDV protein levels in SRBSDV‐infected plants determined by western blotting. (d) Viral incidence in NIP (non‐transformed controls) and OEP10‐10 plants. The numbers of healthy and diseased plants in each treatment were determined by RT‐PCR at 30 days post‐inoculation. Each treatment used at least 30 seedlings, and three biological replicates were performed. Error bars represent ± standard deviation (SD). An asterisk at the top of a column indicates significant difference at P < 0.05.