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. 2019 Feb 14;20(5):641–655. doi: 10.1111/mpp.12782

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© 2019 The Authors. Molecular Plant Pathology Published by British Society for Plant Pathology and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The effect of Rice stripe virus (RSV) infection on OEP10 transgenic plants expressing the Rice black‐streaked dwarf virus (RBSDV) P10 protein. (a) The visual appearance of mock‐ and RSV‐infected plants. (b) Viral incidence in NIP (non‐transformed controls) and OEP10‐10 plants. The numbers of healthy and diseased plants in each treatment were determined by reverse transcription‐polymerase chain reaction (RT‐PCR) at 30 days post‐inoculation. Each treatment used at least 30 seedlings, and three biological replicates were performed. (c) The relative expression levels of the RSV coat protein (CP) gene in RSV‐infected plants assessed by quantitative RT‐PCR. RSV‐CP1 and RSV‐CP2 are two pairs of primers designed at different positions in the RSV CP gene. (d) The RSV CP protein levels in RSV‐infected plants determined by western blotting. Error bars represent ± standard deviation (SD). An asterisk at the top of a column indicates a significant difference at P < 0.05.