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. 2019 Jul 11;10:1605. doi: 10.3389/fmicb.2019.01605

FIGURE 5.

FIGURE 5

Topoisomerase I depletion-induced changes in the transcription of genes involved in sporulation regulatory cascades. (A) RNA-Seq based expression of selected genes encoding for whi and bld regulatory cascades genes normalized by the upper quartile in the wild type strain (black) and the TopA-depleted strain (red). The genes belonging to the BldD regulon are marked with arrows. The statistically significant changes in transcript levels were marked with asterisks (∗p-value <0.05; ∗∗∗p-value <0.001). p-Values were calculated based on RNA-Seq data analysis for four independent biological replicates for the wild type and topA-downregulated strains. (B) RT-qPCR analysis of whiG transcript levels at different concentrations of the topA gene inducer. Wild type levels of whiG transcription were set at 1, and standard deviation for wild type transcription is marked with a gray bar. The inset shows RT-qPCR analysis of whiG transcript levels in the TopA-depleted and the wild type strains, as well as the control strain that contained the pIJ6902 plasmid without the topA gene. The whiG transcript enrichment was normalized by the expression of the hrdB gene which served as the endogenous control and compared to the wild type strain in which transcription was estimated as 1.