Skip to main content
. Author manuscript; available in PMC: 2019 Jul 18.
Published in final edited form as: Nat Protoc. 2019 Jan;14(1):1–27. doi: 10.1038/s41596-018-0069-7

Fig. 3 |. Overview of HIV-1 virus preparation.

Fig. 3 |

HEK293T cells are plated and transfected with an HIV-1 molecular clone, using appropriate biosafety precautions. The virus-containing supernatant is harvested 48 and 72 h after transfection. PEG precipitation, followed by centrifugation, is implemented to pellet the virus for subsequent concentration, titering, and storage.