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. Author manuscript; available in PMC: 2019 Jul 18.
Published in final edited form as: Nat Protoc. 2019 Jan;14(1):1–27. doi: 10.1038/s41596-018-0069-7
Cycle no. Denature Anneal Extend
1 98 °C, 5 min
2–15 98 °C, 30 s 65 °C, −0.5 °C per cycle, 20 s 72 °C, 1 min
16–35 98 °C, 30 s 58 °C, 20 s 72 °C, 1 min
36 72 °C, 10 min
End