Figure 3.

LDGs originated from in vitro M. tuberculosis infection released a high level of NETs. Freshly collected whole blood from healthy controls was infected with M. tuberculosis for 2 h and centrifuged by Ficoll density gradient centrifugation. LDGs in the PBMCs were identified as CD14^low CD15⁺ cells and enumerated by FCM. Data are represented as mean ± SD of at least three independent experiments and the statistical significance was determined by unpaired t-test (*p < 0.05) (A). NDGs from the RBC fraction and LDGs from the PBMC fraction were isolated individually by the immunomagnetic negative selective method, stained with anti-MPO (green) antibody and DAPI (blue), and observed under a fluorescence microscope (B) (×200).