Figure 6.

The level of ROS regulates the generation of LDG. NDGs were isolated from healthy controls and either untreated or pretreated with NAC (30 μM) or H₂O₂ (50 μM) for 30 min, and then, infected with or without M. tuberculosis for 2 h. (A) Cells were loaded with H2DCF-DA fluorescence probe (10 μM), and the intracellular ROS levels were determined by FCM. (B) The generation of LDGs was detected as described above. (C,D) NDGs were separated from healthy controls and pretreated with or without DPI (25 μM) for 30 min, followed by infection with M. tuberculosis for 2 h. The intracellular ROS levels in neutrophils and generation of LDNs were detected as described above. The results are expressed as mean ± SD of at least three independent experiments and the statistical significance was determined by one-way ANOVA and post hoc test (*p < 0.05).