FIGURE 11.

In vivo effect of melatonin on the radiation-induced actions on the VEGF-stimulated angiogenesis in the chorioallantoic membrane assay. Eggs were irradiated and alginate beads with VEGF 1.57 pM, as stimulator of blood vessel formation, with and without melatonin 1 mM, were placed on the chorioallantoic membrane on day 11 of development. On day 14, newly formed blood vessel converging toward the alginates were counted at microscopic level. (A) Representative images of CAM. (B) Vessel number data shown are expressed as mean ± SEM. C, control; M, melatonin; VEGF, vascular endothelial growth factor; and R, radiation. a, p < 0.001 vs C; b, p < 0.05 vs C; c, p < 0.001 vs VEGF; and d, p < 0.001 vs R.