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. 2019 Jul 11;10:879. doi: 10.3389/fphys.2019.00879

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Copyright © 2019 González-González, González, Rueda, Alonso-González, Menéndez-Menéndez, Gómez-Arozamena, Martínez-Campa and Cos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Potentiation by melatonin (M) of radiation (R)-induced effects on HUVEC capillary structure formation. HUVECs control (C) and melatonin pretreated cells for a week were seeded on growth factor-reduced BME in medium supplemented with 2% FBS and irradiated. After 4 h of incubation, tubular structures were photographed and tubule area was measured as described in see section “Material and Methods.” Tubule area data are expressed as the percentage of the control group (mean ± SEM). a, p < 0.001 vs C (control); and b, p < 0.05 vs R.