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. 2017 Mar 12;19(2):405–417. doi: 10.1111/mpp.12532

Figure 3.

Figure 3

The accumulation of viral coat protein in NbTRXh2‐knockdown plants or protoplasts. Total proteins were extracted from Barley mosaic virus (BaMV)‐inoculated Luc‐ or NbTRXh2‐knockdown plants in (A) and protoplasts in (D), and subjected to Western blot analysis. The accumulation levels of BaMV coat protein in Luc‐knockdown plants at 5 days post‐inoculation (dpi) in (A) and in Luc‐knockdown protoplasts at 48 hpi in (D) were set as 100%. (B) The total RNAs were extracted from the knockdown plants as indicated and used to determine the knockdown efficiency by real‐time reverse transcription‐polymerase chain reaction (RT‐PCR). The numbers shown above the bars are the average and standard error of the relative expression of NbTRXh2 from at least three independent experiments with at least two plants for each experiment. (C) The accumulation levels of Potato virus X (PVX) in Luc‐ and NbTRXh2‐knockdown Nicotiana benthamiana leaves at 7 dpi were also analysed by Western blot analysis. The coat protein accumulation levels of each virus in the Luc‐knockdown control plants were set as 100%. The numbers are the average levels of coat protein with the standard deviation in (A), (C) and (D) obtained from at least three independent experiments with a total of 5–7 plants for each treatment. Luc, Luciferase‐knockdown plants; NbTRXh2, NbTRXh2‐knockdown plants; cp, coat protein; rbcL, RuBisCo large subunit (the loading control for normalization). Asterisks indicate a statistically significant difference of the indicated group analysed by Student's t‐test (*P < 0.05, ***P < 0.001).