Figure 5.

Localization of NbTRXh2‐OFP and its derivatives in Nicotiana benthamiana cells. Orange fluorescent protein (OFP), NbTRXh2‐OFP and NbTRXh2/G2A‐OFP were transiently expressed by agro‐infiltration in N. benthamiana plants (A) and in protoplasts (B) and detected by an Olympus Fluoview FV1000 confocal microscope with 543‐nm laser excitation. DIC, differential interference contrast. (C) OFP, NbTRXh2‐OFP and the two mutants NbTRXh2/G2A‐OFP and NbTRXh2/C62A‐OFP in the soluble (S) or membrane (M) fraction were detected by Western blot analysis. The primary antibody was the rabbit anti‐OFP antibody, and the secondary antibody was fluorescently labelled goat anti‐rabbit immunoglobulin G (IgG) antibody. The molecular weight of the protein bands is indicated to the left of the gel.