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. 2018 Feb 1;19(7):1595–1611. doi: 10.1111/mpp.12633

Figure 4.

Figure 4

FgLetm1 is localized to the mitochondria and critical for mitochondrial integrity. (a) Both FgLetm1 and FgLetm2 are localized to the mitochondria. Mycelia of FgLetm1‐C and FgLetm2‐C were grown in Completed medium (CM) and stained with Mito‐HcRed. Images were taken by a confocal fluorescence microscope. Bar, 10 µm. DIC, Differential Interference Contrast (b) ΔFgLetm1 changed the mitochondrial structural patterns. Strains were grown in CM broth for 16 h at 25 °C, harvested and stained with Mito‐HcRed for observation. Typical patterns in individual strains are shown. Bar, 10 µm. (c) The ΔFgLetm1 mutant caused mitochondrial swelling. The ultrastructural morphology of mitochondria in each strain was visualized by transmission electron microscopy. Bar dimensions are shown in the images. (d) ΔFgLetm1 decreased the protein level of cytochrome b (Cyt b), an indicator protein of respiratory chain components. The protein abundance of Cyt b in PH‐1 and the mutants were analysed by immunoblot assays. Histone H3 was used as a reference protein.