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The discrete chlorotic spots produced by D28E+8 in dip‐inoculated Nicotiana benthamiana are dependent on coronafacic acid (CFA). D28E+2 and D28E+8 derivatives with and without the cfa operon were dip inoculated into N. benthamiana leaves at 105 CFU/mL, which were then photographed at 6 days post‐inoculation (dpi). Three plants were inoculated per strain, and representative leaves are shown. The experiment was repeated twice with similar results.
