Figure 3.

Coronafacic acid (CFA), coronamic acid (CMA) and coronatine (COR) do not contribute significantly to the growth of D28E+2 or D28E+8 in dip‐ or syringe‐inoculated Nicotiana benthamiana leaves. (A) Plants were dipped with the indicated strains at 10⁵ colony‐forming units (CFU)/mL and bacterial populations in leaf tissue at 6 days post‐inoculation (dpi) were determined by plate counting. The chart shows the combined data from five independent experiments, with each experiment containing different subsets of the strains shown. Each strain was evaluated in at least two independent experiments, with three plants per strain in every experiment. (B) Leaves were inoculated by blunt syringe pressure infiltration with the indicated strains at 10⁴ CFU/mL, and bacterial populations were measured at 6 dpi. The chart shows combined data from seven independent experiments, with each experiment containing different subsets of the strains shown. Each strain was evaluated in at least three independent experiments, with three plants per strain in every experiment. Growth is depicted as the least‐squares means with standard error (SE) of log(CFU/cm²). Means marked with the same letter are not significantly different using the Tukey honestly significant difference (HSD) method of multiple sample comparison (α = 0.05).