Figure 5.

The method of inoculation and level of inoculum strongly influence the nature of the symptoms produced by D28E+2+cmaL and D28E+8+cmaL in Nicotiana benthamiana. (A, B) Plants were dip inoculated (A) or vacuum infiltrated (B) with D28E+2+cmaL at the indicated levels of inoculum, and the plants were photographed at 7 days post‐inoculation (dpi) (dip) or 6 dpi (vacuum). Representative leaves from three plants per treatment are shown. It should be noted that all leaves were of equivalent size when inoculated, and similar results were observed in an independent experiment. (C) Zones in N. benthamiana leaves were inoculated by syringe infiltration with c. 50 µL of bacterial suspension at the indicated colony‐forming units (CFU)/mL and photographed at 7 dpi. Black marks on the leaves were used to delineate the perimeter of the infiltration zones, which are further outlined here in red and yellow for inoculations at 10³ and 10² CFU/mL, respectively. Inoculations were similarly performed in three leaves, with a representative leaf shown, and the experiment was repeated twice with similar results.